WDR43 mutations were not only found to cause defects in craniofacial development in zebrafish, but were also associated with the occurrence of human estrogen receptor-negative breast cancer. Studies have shown that WDR43 can suspend the release of Pol II and promote transcription extension to regulate the high-level transcription and translation process in the pluripotency of embryonic stem cells. It is usually involved in the assembly of small-subunit processome as a conserved component, and mediates the transcription and processing of small-subunit ribosomal RNA (18S rRNA), which plays a key role in the biogenesis of ribosomes. The WDR43 protein belongs to the protein family containing the WDR domain. Proteins containing the WDR domain participate in a wide range of cellular networks, such as signal transduction, transcriptional regulation, cell cycle control, cytoskeleton assembly and chromatin modification, many of which are associated with human diseases. It is usually used as a scaffold for protein interaction and provides a platform for the assembly of multi-protein complexes. The WD40 repeat (WDR) domain is a domain composed of multiple β-helical structures. Therefore, it is very important to identify novel targets for the treatment and diagnosis of CRC. Patients with distant metastases generally have a relatively poor prognosis, due to accelerated tumor spreading and resistance to chemotherapy and radiotherapy, which makes CRC a difficult clinical challenge. In China, despite the widespread use of early screening for individuals with a high-risk family history, most patients are diagnosed at the late stage of the disease and cannot benefit from surgical treatment. Particularly in developing countries, the incidence of CRC is gradually increasing.
WDR43 may serve as a valuable biomarker and provide new options for the diagnosis and treatment of colorectal cancer.Ĭolorectal cancer (CRC) accounts for 10% of the total cases of cancer and cancer-related mortality worldwide per annum. In conclusion, the role of WDR43 in the occurrence and development of CRC was investigated in the present study. In addition, it was found that WDR43 knockdown inhibited vimentin (VIM) expression in CRC cells and overexpression of VIM can partially reverse the effects of WDR43 both in vitro and in vivo. WDR43 knockdown was shown to increase apoptosis and inhibit the proliferation, migration and invasion of CRC cells in vitro and reduce tumorigenesis in animal models. Immunohistochemistry of 16 patient specimens confirmed that the expression of WDR43 was elevated in CRC. Finally, we explored potential downstream proteins or pathways and established subcutaneous xenograft model to verify our findings. Subsequently, the high expression of WDR43 in human clinical samples of CRC was validated and we further examined the biological functions of it in CRC cells. In the present study, we searched the TCGA database and found the correlation between WDR43 and CRC. Its biological function is largely unclear, particularly in colorectal cancer (CRC). WD40 repeat (WDR)43 is an RNA-binding protein that belongs to the WDR domain protein family.
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